Flipr calcium 5 assay kit manual

FLIPR Calcium 5 Assay Kit. The FLIPR Calcium 5 Assay Kit utilizes a unique fluorophore that exhibits a large signal window, and is amenable to incubation at 37°C or room temperature, facilitating fully-automated screens that may run overnight. * Each reagent vial (Component A) is sufficient for 1 plate (, , well). the flipr tetra system, together with screenworks peak pro software and the flipr calcium 5 assay kit, provide a complete, integrated solution for directing your drug design by allowing you to eliminate cardiotoxic compounds and identify potential cardiac drug candidates earlier in the drug discovery process. 1. This is sufficient reagent to assay one entire plate. Once mixed, the Calcium Assay Buffer (10X) - (Item No. ) The vial contains 5 ml of concentrated Assay Buffer (1M Tris-HCl, pH ). Dilute the entire contents of the vial with 45 ml of HPLC grade water. The diluted buffer ( mM Tris-HCl, pH ) is used to prepare the calcium.

Calcium 5 Kit no PBX Ca6+PBX Ca6 (no PBX) Ca5+PBX Ca5 (no PBX) EC 50 (nM) 13 39 14 ND Z @ EC 80 ND CHO-M1 cells in media: probenecid comparison Log [carbachol] ˜M Δ F/F (max-min) FLIPR Calcium 5 Assay Kit uses a different formulation enabling shorter incubation time FLIPR Calcium 5 Assay Kit signal window comparison in hTRPV1. The FLIPR Calcium 6 Assay Kit and FLIPR Calcium 6QF Assay Kit from Molecular Devices provide a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium. This kit is based on a novel calcium indicator that provides a larger signal window. The Calcium 6QF formulation is a flexible option for quench. FLIPR® Calcium 4 and Calcium 5 Assay Kit Protocol: FLIPR® Calcium 5 Explorer Kit (Molecular Devices, Inc.) includes 10 vials of dye (Component A) and 1 bottle of Hanks Balanced Salt Solution (HBSS) and 20 mM HEPES adjusted to pH (Component B) sufficient for 1 plate each. Bulk kits m contain 10 vials of dye sufficient for 10 plates each.

Universal mix-and-read protocol accelerates assay workflow and increases throughput. Superior signal-to-noise ratio facilitates confirmation of endogenous or. The fluorescent imaging plate reader (FLIPR) Calcium 3 Assay Kit, optimal for targets that have proteins or peptides as agonists, has 2 potential drawbacks. Each product is shipped with documentation stating specifications and other technical information. Molecular Devices products are warranted to meet the stated.