Dh5 alpha invitrogen manual

Description. Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of 1 x 10 6 cfu/μg plasmid DNA. Designed for general, everyday use. Contain genetic markers useful for general applications. Not high-throughput compatible (Manual)Brand: InvitrogenTrade. GenScript Corporation Tel: Fax: www.doorway.ru email: info@www.doorway.ru DH5α Competent Cells Cat. No. M Technical Manual No. Version NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. High efficiency strain ideal for a wide variety of applications. Available in a wide variety of sizes, including single-use vials, μl vials, and well plate and 12 x 8 tube strips.

Library Efficiency® DH5 is available from Invitrogen (Catalog no. ). Page 2 Transformation Procedure pUC19 control DNA (10 pg/µl) is provided to check. Subcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield 1 x 10 6 transformants/µg control DNA per 50 µL reaction. For Research Use Only. Not for use in diagnostic procedures. 1. Thaw a tube of DH5 alpha Competent E. coli cells on ice. 2. Add µl containing 1 pg ng of plasmid DNA to the cell mixture. Carefully flick the tube times to mix cells and DNA. Do not vortex. 3. Place the mixture on ice for 2 minutes. Do not mix. 4. Heat shock at exactly 42°C for exactly 30 seconds. Do not mix. 5. Place on ice for 2 minutes.

The instructions provided below are for general use. Plasmid DNA should be free of Visit the Invitrogen Web Resource using your World Wide Web browser. Review and cite DH5 ALPHA CELLS protocol, troubleshooting and other newer TOPO TA cloning kits from invitrogen for transforming into DH5 alpha cells. Nov Transformation is the process by which foreign DNA is introduced into a cell. Transformation of bacteria with plasmids is important not only for.